Sains Malaysiana 53(10)(2024): 3405-3416

http://doi.org/10.17576/jsm-2024-5310-14

 

Penghasilan Lipid Kaya Asid Dokosaheksaenoik (DHA) oleh Aurantiochytrium sp. SW1 menggunakan Sisa Kulit Nanas sebagai Sumber Karbon Alternatif

(Docosahexaenoic Acid (DHA)-Rich Lipid Production by Aurantiochytrium sp. SW1 using Pineapple Peel Waste as an Alternative Carbon Source)

 

AIMAN DANIAL MOHD ZAINI¹, ASVITRA PREVENA PALANIANDY¹, MUHAMMAD ZIYAD ISMAIL², NURUL AQILAH MOHD ZAINI¹, NURFATIMAH MOHD THANI¹, MOHAMAD YUSOF MASKAT¹, AIDIL ABDUL HAMID² & MOHAMED YUSUF MOHAMED NAZIR^1,3,*

 

¹Department of Food Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

²Department of Biological Sciences & Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

³Innovation Centre for Confectionery Technology (MANIS), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

 

Received: 29 February 2024/Accepted: 28 August 2024

 

Abstrak

Thraustokaitrid seperti Aurantiochytrium sp. telah menarik perhatian penyelidik dan industri disebabkan kebolehannya untuk menghasilkan asid lemak politaktepu (PUFA) bernilai tinggi terutamanya asid dokosaheksaenoik (DHA, C22:6 ω-3). Namun begitu, keperluan penggunaan substrat yang mahal khususnya sumber karbon tulen semasa proses pengkulturan telah menyumbang kepada peningkatan kos pengkomersialan DHA daripada thraustokaitrid. Oleh itu, dalam kajian ini, potensi penggunaan sisa kulit nanas (SKN) yang merupakan antara sisa utama industri pertanian di Malaysia sebagai sumber karbon alternatif yang lebih murah untuk penghasilan DHA daripada Aurantiochytrium sp. SW1 telah dikaji. Memandangkan SKN mengandungi lignin, selulosa dan hemiselulosa yang agak kompleks, kaedah perawatan autoklaf, berasid dan beralkali pada kepekatan berbeza telah ditentukan bagi mendapatkan gula jumlah yang optimum daripada SKN. Hasil menunjukkan perawatan autoklaf secara signifikan (p<0.05) menghasilkan hidrolisat dengan kepekatan gula tertinggi (39.6 ± 1.98 g/L) berbanding dengan perawatan berasid dan alkali yang masing-masing dapat mengekstrak dalam julat 31-38 g/L gula jumlah. Kemudian, kebolehan Aurantiochytrium sp. SW1 untuk menggunakan kesemua hidrolisat SKN sebagai sumber karbon alternatif dibandingkan. Didapati, penggunaan hidrolisat SKN dengan rawatan 100 mM H₂SO₄ bersama nutrien tambahan secara signifikan (p<0.05) menghasilkan biojisim tertinggi (7.74 g/L ± 0.39) manakala kandungan lipid terbaik (60.7% ± 3.04) terhasil apabila SW1 dikulturkan menggunakan hidrolisat SKN dengan rawatan autoklaf tanpa penambahan nutrien. Penghasilan DHA yang maksimum (0.68 ± 0.034 g/L) dicapai apabila Aurantiochytrium sp. SW1 dikultur menggunakan hidrolisat SKN dengan rawatan 100 mM H₂SO₄ bersama nutrien tambahan yang mana 10-35% lebih tinggi jika dibandingkan dengan penghasilan menggunakan hidrolisat SKN yang lain. Kajian ini dapat menjadi asas ke arah penghasilan DHA yang lebih murah daripada thraustokaitrid dengan menggunakan SKN sebagai sumber karbon alternatif.

 

Kata kunci: Asid dokosaheksaenoik; Aurantiochytrium sp.; sisa kulit nanas; teknik rawatan

 

Abstract

Thraustochytrids such as Aurantiochytrium sp. have drawn the attention of researchers and industries due to their ability to produce high-value polyunsaturated fatty acids (PUFAs), especially docosahexaenoic acid (DHA, C22:6 ω-3) from its total fatty acids. However, the requirement for expensive substrates, particularly refined carbon sources during the cultivation process, has contributed to the increased commercialization costs of DHA from thraustochytrids. Therefore, in this study, the potential use of pineapple peel waste (PPW), which is among the major agricultural wastes in Malaysia, as a cheaper alternative carbon source for DHA production from Aurantiochytrium sp. SW1 was investigated. Considering that PPW contains relatively complex lignin, cellulose, and hemicellulose, autoclave, acidic, and alkaline treatment methods at different concentrations were examined to obtain the optimal sugar content from PPW. The results showed that autoclave treatment yielded the highest sugar concentration (39.6 ± 0.39 g/L) significantly (p<0.05), compared to acidic and alkaline treatments, each of which could extract sugar within the range of 31-38 g/L. Subsequently, the ability of Aurantiochytrium sp. SW1 to utilize all PPW hydrolysates as a sole carbon source was compared. It was found that the use of PPW hydrolysate with 100 mM H₂SO₄ treatment along with additional nutrients produced the maximum biomass (7.74 ± 0.39 g/L) significantly (p<0.05), and outstanding lipid content (60.7 ± 3.04%) was obtained when SW1 was cultured using autoclaved PPW hydrolysate without nutrient supplementation. The maximum DHA production (0.68 ± 0.034 g/L) was achieved when Aurantiochytrium sp. SW1 was cultured using PPW hydrolysate with 100 mM H₂SO₄ treatment along with additional nutrients, which was 10-35% higher compared to production using other PPW hydrolysates. This study could serve as a foundation towards cheaper DHA production from thraustochytrids using PPW as an alternative carbon source.

 

Keywords: Aurantiochytrium sp.; docosahexaenoic acids; pineapple peel waste; treatment methods

 

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*Corresponding author; email: yusufnazir@ukm.edu.my